Fecal microbiota transplantation plus pembrolizumab and axitinib in metastatic renal cell carcinoma: the randomized phase 2 TACITO trial.

Journal: Nature medicine

This phase 2a TACITO trial tested whether adding fecal microbiota transplantation (FMT) from “super-responder” donors to standard first-line pembrolizumab plus axitinib improves outcomes in treatment-naive metastatic renal cell carcinoma.

Key design points

  • Investigator-initiated, randomized, double-blind, placebo-controlled.
  • Patients: 45 with metastatic RCC, no prior systemic therapy.
  • Arms:
  •     Donor FMT (d-FMT) + pembrolizumab/axitinib
  •     Placebo FMT (p-FMT) + pembrolizumab/axitinib
  • FMT donors were patients with complete responses to immune checkpoint inhibitors.

Efficacy

  • Primary endpoint (12‑month progression‑free survival rate) was numerically improved but not statistically significant:
  •     70% with d-FMT vs 41% with p-FMT (P = 0.053).
  • Secondary endpoints favored d-FMT:
  •     Median PFS: 24.0 months (d-FMT) vs 9.0 months (p-FMT); HR = 0.50, P = 0.035.
  •     Objective response rate: 52% (d-FMT) vs 32% (p-FMT).
  • Overall survival was a secondary endpoint but is not detailed in the abstract.

Microbiome findings

  • Donor strains successfully engrafted in recipients.
  • d-FMT increased alpha diversity and induced larger compositional shifts (beta diversity) versus baseline.
  • Clinical benefit correlated with acquisition or loss of specific strains rather than overall engraftment magnitude.

Safety

  • The abstract reports FMT as safe in this setting; no specific new safety signals are described.

Clinical interpretation

  • Although the formal primary endpoint was narrowly missed, the magnitude of PFS improvement and higher response rate suggest a clinically meaningful signal that modulating the gut microbiome with carefully selected donor FMT may enhance ICI-based therapy in metastatic RCC.
  • Results are hypothesis‑generating and support larger confirmatory studies and further work to define the key bacterial strains driving benefit.

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